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2 edition of Isolation and characterisation of mutants of cowpea mosaic virus found in the catalog.

Isolation and characterisation of mutants of cowpea mosaic virus

Claire Louise Lesley Holness

Isolation and characterisation of mutants of cowpea mosaic virus

by Claire Louise Lesley Holness

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Published by typescript in [s.l.] .
Written in English


Edition Notes

Thesis (Ph.D.) - University of Warwick, 1989.

StatementClarie Louise Lesley Holness.
ID Numbers
Open LibraryOL13939202M

Characterization and Electron Microscopy of a Potyvirus Infecting Commelina diffusa. F. J. Morales, F. W. Zettler. Pages VIEW ABSTRACT | VIEW ARTICLE. Etiology. Isolation and Some Properties of a Yellow Subgroup Member of Cowpea Mosaic Virus from Illinois. M. R. McLaughlin, Pornpod Thongmeearkom, G. M. Milbrath and Robert M. Goodman. Mosaic viruses are plant viruses that cause the leaves to have a speckled appearance. Mosaic virus is not a taxon.. As of March species include.

The present study aimed to evaluate some plant extracts from Plectranthus tenuiflorus, Azadirachta indica, Clerodendrum inerme, Schinus terebinthifolius and Mirabilis jalapa as antiviral materials against Bean common mosaic virus (BCMV) in bean plants. The results showed that all the tested plant extracts were effective in reducing the number of local lesions formed by BCMV. Cowpea mottle virus is of localized importance on cowpea in Nigeria. Cucumber mosaic virus (CMV) is worldwide in distribution. The virus causing cucumber mosaic has a wider range of hosts and attacks a greater variety of vegetables, ornamentals, weeds, and other plants than other viruses. In view of the fact that mixed infections involving.

Serological Grouping of Isolates of Blackeye Cowpea Mosaic and Cowpea Aphidborne Mosaic Viruses. M. A. Taiwo, D. Gonsalves. Pages VIEW ABSTRACT | VIEW ARTICLE. Physiology and Biochemistry. Partial Characterization and Grouping of Isolates of Blackeye Cowpea Mosaic and Cowpea Aphidborne Mosaic Viruses. M. A. The presence of high molecular weight double-stranded RNA (dsRNA) within plant cells is an indicator of infection with RNA viruses as these possess genomic or replicative dsRNA. DECS (dsRNA isolation, exhaustive amplification, cloning, and sequencing) analysis has been shown to be capable of detecting unknown viruses. We postulated that a combination of DECS analysis and next-generation.


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Isolation and characterisation of mutants of cowpea mosaic virus by Claire Louise Lesley Holness Download PDF EPUB FB2

A nitrous acid-induced, temperature sensitive mutant of cowpea mosaic virus (CPMV) known as(EvansVirology, ), was characterised. The phenotypic defect in 8 was shown not to affect translation of the RNA or the first proteolytic cleavage of the B RNA-encoded polyprotein. The defect is probably at the level of genome replication.

Isolation and characterization of two temperature-sensitive mutants of cowpea mosaic virus. Evans D(1). Author information: (1)John Innes Institute, Colney Lane, Norwich NR4 7UH, England. Two temperature-sensitive mutants of cowpea mosaic virus have been isolated following nitrous acid by: 1.

VIROLOGY() Isolation and Characterization of Two Temperature-Sensitive Mutants of Cowpea Mosaic Virus D. EVANS1 John Inn Institute, Colney Lane, Norwich NB4 7UH, England Received September h, l9Sh; accepted, Octo i Two temperature-sensitive mutants of cowpea mosaic virus have been isolated following nitrous acid by: 1.

Isolation and characterisation of two temperature-sensitive mutants of cowpea mosaic virus. Isopycnic centrifugation of plant viruses in Nycodenz density : Claire Louise Lesley Holness. A number of mutants of CPMV have been isolated following nitrous acid mutagenesis of virion RNA.

This paper describes the characterization of a mutant which is restricted in its ability to replicate in cowpeas. The hosts used in this study were two varieties of cowpea and Phaseolus vulgaris vat.

'Pinto'. Two temperature-sensitive mutants of cowpea mosaic virus have been isolated following nitrous acid mutagenesis. One mutant,was shown to have a temperature-sensitive defect in the B component RNA. The location of the temperature-sensitive defect of the other mutant,was not unambiguously determined.

Recent results with tobacco mosaic virus help to overcome this difficulty. The infective unit of the virus is its ribonucleic acid component, which can be isolated in active form 1.

To avoid the present confusion in virus names of the Group 2, the authors proposed to use a name of cowpea aphid-borne mosaic virus (CAMV) (Lovisolo and Conti, ) for all members of the Group 2. The remaining two viruses isolated from cowpea plants were cucumber mosaic virus (CMV) and subclover mottle virus.

Cowpea mosaic virus (CPMV) is a picorna-like plant virus. As well as an intrinsic interest in CPMV as a plant pathogen, CPMV is of major interest in biotechnology applications such as nanotechnology.

Two temperature-sensitive mutants of cowpea mosaic virus have been isolated following nitrous acid mutagenesis. One mutant,was shown to have a temperature-sensitive defect in the B component RNA. The location of the temperature-sensitive defect of the other mutant,was not unambiguously determined.

No virus-specific proteins were detected in leaves inoculated with the mutants and. Different mutants of Cowpea Mosaic Virus (CPMV) have been used as scaffolds to bind 2 and 5 nm gold nanoparticles through gold−sulfur bond formation at specific locations on the virus to produce patterns of specific interparticle distances.

TEM images confirm that the bound gold particles produce patterns of gold nanoparticles that correlate well with models built from the known locations of.

Cauliflower mosaic virus (CaMV) encodes a movement protein (MP) which forms tubules in vivo and mediates the translocation of virus particles through plasmodesmata. The relationship between CaMV MP structure and function, in isolation from the complete virus infection, was studied by using MP expression in insect cells.

Abstract The isolation and characterization of replication-deficient, temperature-sensitive (ts) mutants of Cowpea chlorotic mottle virus (CCMV) are described. The mutants multiply in cowpea and soybean at 25° but not at 35°.

One group of mutants maps on CCMV RNA component 3, and the rest map on RNA component 1. Cowpea mosaic virus is among the most economically important viruses infecting cowpea. Incidence of infection was assessed in two governorates in Egypt, namely Alexandria and El Beheira.

Evidence is presented for a previously undiscovered protein that is associated with the virion of cowpea mosaic virus (CPMV).

It co‐purified with the viral RNA. Isolation and characterization of two temperature-sensitive mutants of cowpea mosaic virus, Virology, /(85),2, (), ().

Cowpea mosaic virus (CPMV) is a non-enveloped plant virus of the comovirus group. Infection of a susceptible cowpea leaf causes a "mosaic" pattern in the leaf, and results in high virus yields ( g/kg).Its genome consists of 2 molecules of positive-sense RNA (RNA-1 and RNA-2) which are separately encapsidated.

Both RNA1 and RNA2 have a VPg (virus genome-linked protein) at the. Two temperature-sensitive mutants of cowpea mosaic virus have been isolated following nitrous acid mutagenesis.

One mutant,was shown to have a temperature-sensitive defect in the B. The cowpea mosaic virus is transmitted by Aphis rumicis L., Macrosiphum pisi Kalt. and Aphis gossypii Glov.

It is also transmitted by artificial juice inoculation. The virus is seed‐bome. It withstands ageing m vitro for 3 days at C. Its inactivation temperature is approximately 62°.

In addition, several Arabidopsis mutants in which virus movement is restricted have been identified: these include cum1‐1 and cum2‐1, which affect the local spread of Cucumber mosaic virus (CMV) (Yoshii et al., a) and that of CMV and Turnip crinkle virus (TCV) (Yoshii et al., b), respectively, and vsm1, in which the systemic.

Synthesis and Characterization of Iron Oxide Derivatized Mutant Cowpea Mosaic Virus Hybrid Nanoparticles. Advanced Materials20 (24), DOI: /adma. Zoeten GA, et al. Association of cowpea mosaic virus-induced double-stranded RNA with a cytopathological structure in infected cells.

VirologyPubMed: De Jager CP. Genetic analysis of cowpea mosaic virus mutants by supplementation and reassortment. VirologyPubMed: Movement-deficient potato virus X (PVX) mutants tagged with the green fluorescent protein were used to investigate the role of the coat protein (CP) and triple gene block (TGB) proteins in virus movement.

Mutants lacking either a functional CP or TGB were restricted to single epidermal cells. Microinjection of dextran probes into cells infected with the mutants showed that an increase in the.Cucumber mosaic virus (CMV) is transmitted by aphid vectors and the coat protein (CP) is a primary determinant of transmission.

The stability of the aphid transmission phenotype of transmissible field isolates of CMV was evaluated using Aphis gossypii and Myzus persicae. All isolates were transmitted, with A.

gossypii generally showing a higher efficiency of transmission than M. persicae.